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URN: urn:nbn:de:bvb:20-opus-68810
URL: http://opus.bibliothek.uni-wuerzburg.de/volltexte/2012/6881/
LgR5 expression and cancer stem cell hypothesis: clue to define the true origin of esophageal adenocarcinomas with and without Barrett's Esophagus?
von Rahden, Burkhard H. A. ;
Kircher, Stefan ;
Lazariotou, Maria ;
Reiber, Christoph ;
Stuermer, Luisa ;
Otto, Christoph ;
Germer, Christoph T. ;
Grimm, Martin
| Originalveröffentlichung: |
| (2011) In: Journal of Experimental & Clinical Cancer Research (2011) 30:23, doi: 10.1186/1756-9966-30-23 |
| pdf-Format:
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| SWD-Schlagwörter: |
| Medizin |
| Institut: |
| Chirurgische Klinik und Poliklinik |
| DDC-Sachgruppe: |
| Medizin |
| Dokumentart: |
| Aufsatz / Artikel |
| Sprache: |
| Englisch |
| Erstellungsjahr: |
| 2011 |
| Publikationsdatum: |
| 15.11.2012 |
| Kurzfassung auf Englisch: |
| Background:
Investigation of the expression of an intestinal stem cell marker in esophageal adenocarcinomas (EAC) with and without Barrett’s Esophagus (BE), with respect to a cancer stem cell (CSC) hypothesis. Materials and methods: Expression of a putative intestinal stem cell marker LgR5 was analyzed in esophageal cancer specimen (n = 70: 41 EAC with BE, 19 EAC without BE, and n = 10 esophageal squamous-cell carcinomas, ESCC) and in the adenocarcinoma cell line OE-33. Ki-67 and Cdx-2 were co-labelled with LgR5 in double staining experiments. Immunhistochemical expression results were confirmed by RT-PCR and correlated with tumor stage and five-year survival rates.
Results:
LgR5was found expressed in 35 of 41 (85%) EAC with BE and in 16 of 19 (81%) EAC without BE. By contrast, LgR5 was not found to be expressed in ESCC. Quantification of immunolabeling showed 15% LgR5+ cells in EAC with BE, 32% LgR5+ cells in adjacent BE and 13% in EAC without BE. Immunofluorescence double staining experiments with LgR5 and Ki-67 revealed a subpopulation (~5%) of proliferating LgR+/Ki-67+ cells. On mRNAlevel, expression of LgR5 was higher in BE in comparison to EAC (p = 0.0159). High levels of LgR5 expression in BE
associated EAC were associated with poorer survival in univariate analysis.
Conclusion:
The stem cell marker LgR5 is expressed in EAC, irrespective of association with BE, and appears to have negative impact on survival. The subset of proliferating LgR5+ cells (<5%) might resemble rapidly cycling
CSCs, which needs to be substantiated in further investigations. |
| Lizenz: |
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Creative Commons - Namensnennung
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