Infection of Human Coronary Artery Endothelial Cells by Group B Streptococcus Contributes to Dysregulation of Apoptosis, Hemostasis, and Innate Immune Responses [Research Article]

  • Early onset sepsis due to group B streptococcus leads to neonatal morbidity, increased mortality, and long-term neurological deficencies. Interaction between septicemic GBS and confluent monolayers of human coronary artery endothelial cells (HCAECs) was analyzed by genome wide expression profiling. In total, 124 genes were differentially expressed (89 upregulated, 35 downregulated) based on a more than 3-fold difference to control HCAEC. Regulated genes are involved in apoptosis, hemostasis, oxidative stress response, infection, and inflammatioEarly onset sepsis due to group B streptococcus leads to neonatal morbidity, increased mortality, and long-term neurological deficencies. Interaction between septicemic GBS and confluent monolayers of human coronary artery endothelial cells (HCAECs) was analyzed by genome wide expression profiling. In total, 124 genes were differentially expressed (89 upregulated, 35 downregulated) based on a more than 3-fold difference to control HCAEC. Regulated genes are involved in apoptosis, hemostasis, oxidative stress response, infection, and inflammation. Regulation of selected genes and proteins identified in the gene array analysis was confirmed by Real-time RT-PCR assay (granulocy te chemotactic protein 2), ELISA (urokinase, cyclooxygenase 2, granulocyte chemotactic protein 1), and western blotting (Heme oxygenase1, BCL2 interacting protein) at various time points between 4 and 24 hours. These results indicate that GBS infection might influence signalling pathways leading to impaired function of the innate immune system and hemorrhagic and inflammatory complications during GBS sepsis.show moreshow less

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Metadaten
Author:Claudia Beyrich, Jürgen Löffler, Anna Kobsar, Christian P. Speer, Susanne Kneitz, Martin Eigenthaler
URN:urn:nbn:de:bvb:20-opus-68834
Document Type:Journal article
Faculties:Institut für Klinische Biochemie und Pathobiochemie
Language:English
Year of Completion:2011
Source:In: Mediators of inflammation (2011), Article ID 971502, 8 p. doi:10.1155/2011/971502
Dewey Decimal Classification:610 Medizin und Gesundheit
GND Keyword:Medizin
Release Date:2012/11/20
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung