TY  - JOUR
A1  - Kramer, Susanne
A1  - Piper, Sophie
A1  - Estevez, Antonio
A1  - Carrington, Mark
T1  - Polycistronic trypanosome mRNAs are a target for the exosome
T2  - Molecular and Biochemical Parasitology
N2  - Eukaryotic cells have several mRNA quality control checkpoints to avoid the production of aberrant proteins. Intron-containing mRNAs are actively degraded by the nuclear exosome, prevented from nuclear exit and, if these systems fail, degraded by the cytoplasmic NMD machinery. Trypanosomes have only two introns. However, they process mRNA5 from long polycistronic precursors by trans-splicing and polycistronic mRNA molecules frequently arise from any missed splice site. Here, we show that RNAi depletion of the trypanosome exosome, but not of the cytoplasmic 5'-3' exoribonuclease XRNA or the NMD helicase UPF1, causes accumulation of oligocistronic mRNA5. We have also revisited the localization of the trypanosome exosome by expressing eYFP-fusion proteins of the exosome subunits RRP44 and RRP6. Both proteins are significantly enriched in the nucleus. Together with published data, our data suggest a major nuclear function of the trypanosome exosome in rRNA, snoRNA and mRNA quality control.
KW  - Trypanosoma brucei
KW  - Exosome
KW  - NMD
KW  - Polycistronic mRNA
KW  - trans-splicing
KW  - Trypanosomes
Y1  - 2016
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/19135
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-191350
VL  - 205
IS  - 1-2
ER  -