TY  - JOUR
A1  - Boriskin, Yu S.
A1  - Desyatskova, R. G.
A1  - Bogomolova, N. N.
A1  - Gorboulev, Valentin G.
T1  - Stability of rubella virus after long-term persistence in human cell line
N2  - Primary infection of HEp-2 cells with rubella virus resulted in non-cytophatic longterm persistent infection. During four years of persistence the virus was produced in sufficient quantities (up to 6 logs PFU/ml) and did not differ from the parental variant in its pathogenicity for BHK-21 or RK-13 cells, or hemagglutinating activity, but formed smaller plaques. Persistent virus preserved the original antigenicity as judged from reciprocal hemagglutination-inhibition or plaque reduction-neutralization tests with polyclonal antisera. Both original and persistent rubella viruses were thermoresistant (T 56° C) and sligthly temperature-sensitive. Clonal analysis revealed presence of ts-mutants among both original and persistent virus clones with different degrees of plating efficiency at 40°/34° C. RNA fingerprinting showed only minor changes in persistent rubella virus.
KW  - Rubella virus
KW  - persistant infection
KW  - cell culture
Y1  - 1986
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/4073
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-46944
ER  -