TY  - JOUR
A1  - Richert, Elisabeth
A1  - Koinzer, Stefan
A1  - Tode, Jan
A1  - Schlott, Kerstin
A1  - Brinkmann, Ralf
A1  - Hillenkamp, Jost
A1  - Klettner, Alexa
A1  - Roider, Johann
T1  - Release of Different Cell Mediators During Retinal Pigment Epithelium Regeneration Following Selective Retina Therapy
T2  - Investigative Ophthalmology & Visual Science
N2  - PURPOSE. To investigate the effect of selective retina therapy (SRT) on the release of AMD-relevant cell mediators, such as matrix metalloproteinases (MMPs), VEGF, and pigment epithelium derived factor (PEDF) using different laser spot sizes and densities. 

METHODS. Porcine RPE-choroid explants were treated with a pulsed 532 nm Nd:YAG laser using (1) large spot sizes, (2) small spot sizes with a high-density (hd) treatment, and (3) small spot sizes with a low-density (1d) treatment. Explains were cultivated in modified Ussing chambers. RPE regeneration and RPE cell death were investigated by calcein-AM staining and immunofluorescence. The MMP release was examined via zymography and immunofluorescence. VEGF and PEDF secretion was analyzed by ELISA. 

RESULTS. During pigment epithelium regeneration (PER), mitosis and RPE cell migration were observed. Four days after SRT (large spot size) the content of active MMP2 increased significantly (P < 0.01). Hd treatment with small spot sizes resulted also in an increase of active MMP2 (P < 0.05). In immunofluorescence explants showed a localized expression of MMP2 within the healing lesions after irradiation. The PEDF level increased significantly (P = 0.01) after SRT with large spot sizes. VEGF secretion decreased significantly (P < 0.05) following SRT with large spot sizes and with hd treatment of small spot sizes. 

CONCLUSIONS. SRT induces a cytokine profile, which may improve the flux across Brach's membrane, slows down progression of early AMD by RPE regeneration, and inhibits the formation of choroidal neovascularization. The cytokine release depends on the size and density of applied laser spots.
KW  - age-related macular degeneration
KW  - matrix metalloproteases
KW  - pigment epithelium derived factor
KW  - retinal pigment epithelium
KW  - selective retina therapy
KW  - vascular endothelial growth factor
Y1  - 2018
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/22616
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-226161
VL  - 59
IS  - 3
ER  -