TY  - JOUR
A1  - Cate, Marie-Sophie
A1  - Gajendra, Sangeetha
A1  - Alsbury, Samantha
A1  - Raabe, Thomas
A1  - Tear, Guy
A1  - Mitchell, Kevin J.
T1  - Mushroom body defect is required in parallel to Netrin for midline axon guidance in Drosophila
T2  - Development
N2  - The outgrowth of many neurons within the central nervous system is initially directed towards or away from the cells lying at the midline. Recent genetic evidence suggests that a simple model of differential sensitivity to the conserved Netrin attractants and Slit repellents is insufficient to explain the guidance of all axons at the midline. In the Drosophila embryonic ventral nerve cord, many axons still cross the midline in the absence of the Netrin genes (NetA and NetB) or their receptor frazzled. Here we show that mutation of mushroom body defect (mud) dramatically enhances the phenotype of Netrin or frazzled mutants, resulting in many more axons failing to cross the midline, although mutations in mud alone have little effect. This suggests that mud, which encodes a microtubule-binding coiled-coil protein homologous to NuMA and LIN-5, is an essential component of a Netrin-independent pathway that acts in parallel to promote midline crossing. We demonstrate that this novel role of Mud in axon guidance is independent of its previously described role in neural precursor development. These studies identify a parallel pathway controlling midline guidance in Drosophila and highlight a novel role for Mud potentially acting downstream of Frizzled to aid axon guidance.
KW  - Drosophila
KW  - Axon guidance
KW  - Midline
KW  - Mud
KW  - NuMA
KW  - LIN-5
KW  - Netrin
Y1  - 2016
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/18977
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-189770
VL  - 143
IS  - 6
ER  -