TY  - JOUR
A1  - Sattler, Janko
A1  - Noster, Janina
A1  - Brunke, Anne
A1  - Plum, Georg
A1  - Wiegel, Pia
A1  - Kurzai, Oliver
A1  - Meis, Jacques F.
A1  - Hamprecht, Axel
T1  - Comparison of two commercially available qPCR kits for the detection of Candida auris
T2  - Journal of Fungi
N2  - Candida auris is an emerging pathogen with resistance to many commonly used antifungal agents. Infections with C. auris require rapid and reliable detection methods to initiate successful medical treatment and contain hospital outbreaks. Conventional identification methods are prone to errors and can lead to misidentifications. PCR-based assays, in turn, can provide reliable results with low turnaround times. However, only limited data are available on the performance of commercially available assays for C. auris detection. In the present study, the two commercially available PCR assays AurisID (OLM, Newcastle Upon Tyne, UK) and Fungiplex Candida Auris RUO Real-Time PCR (Bruker, Bremen, Germany) were challenged with 29 C. auris isolates from all five clades and eight other Candida species as controls. AurisID reliably detected C. auris with a limit of detection (LoD) of 1 genome copies/reaction. However, false positive results were obtained with high DNA amounts of the closely related species C. haemulonii, C. duobushaemulonii and C. pseudohaemulonii. The Fungiplex Candida Auris RUO Real-Time PCR kit detected C. auris with an LoD of 9 copies/reaction. No false positive results were obtained with this assay. In addition, C. auris could also be detected in human blood samples spiked with pure fungal cultures by both kits. In summary, both kits could detect C. auris-DNA at low DNA concentrations but differed slightly in their limits of detection and specificity.
KW  - qPCR
KW  - detection limits
KW  - sensitivity
KW  - strain specificity
KW  - commercial kits
KW  - Candida auris
KW  - Fungiplex Candida Auris
KW  - AurisID
Y1  - 2021
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/22887
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-228879
SN  - 2309-608X
VL  - 7
IS  - 2
ER  -