TY  - JOUR
A1  - Meyer, Till Jasper
A1  - Gerhard-Hartmann, Elena
A1  - Lodes, Nina
A1  - Scherzad, Agmal
A1  - Hagen, Rudolf
A1  - Steinke, Maria
A1  - Hackenberg, Stephan
T1  - Pilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors
T2  - PLoS One
N2  - Background
The entity assignment of salivary gland tumors (SGT) based on histomorphology can be challenging. Raman spectroscopy has been applied to analyze differences in the molecular composition of tissues. The aim of this study was to evaluate the suitability of RS for entity assignment in SGT.

Methods
Raman data were collected in deparaffinized sections of pleomorphic adenomas (PA) and adenoid cystic carcinomas (ACC). Multivariate data and chemometric analysis were completed using the Unscrambler software.

Results
The Raman spectra detected in ACC samples were mostly assigned to nucleic acids, lipids, and amides. In a principal component-based linear discriminant analysis (LDA) 18 of 20 tumor samples were classified correctly.

Conclusion
In this proof of concept study, we show that a reliable SGT diagnosis based on LDA algorithm appears possible, despite variations in the entity-specific mean spectra. However, a standardized workflow for tissue sample preparation, measurement setup, and chemometric algorithms is essential to get reliable results.
KW  - Head and neck cancers
KW  - salivary gland tumors
KW  - salivary glands
KW  - cancers and neoplasms
KW  - malignant tumors
KW  - lipids
KW  - raman spectroscopy
KW  - surgical oncology
Y1  - 2021
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/26473
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-264736
VL  - 16
IS  - 9
ER  -