TY  - JOUR
A1  - Hacker, Jörg
A1  - Ott, Manfred
A1  - Blum, Gabriele
A1  - Marre, Reinhard
A1  - Heesemann, Jürgen
A1  - Tschäpe, Helmut
A1  - Goebel, Werner
T1  - Genetics of Escherichia coli uropathogenicity: Analysis of the O6:K15:H31 isolate 536
N2  - E. coli strain 536 (06: K15: H31) isolated from a case of acute pyelonephritis, expresses S-fimbrial adhesins, P-related fimbriae, common type I fimbriae, and hemolysins. The respective chromosomally encoded determinants were cloned by constructing a genomic library of this strain. Furthermore, the strain produces the iron uptake substance, enterocheline, damages HeLa cells, and behaves in a serum-resistant mode. Genetic analysis of spontaneously arising non-hemolytic variants revealed that some of the virulence genes were physically linked to large unstable DNA regions, termed "pathogenicity islands", which were mapped in the respective positions on the E. coli K-12linkage map. By comparing the wild type strain and mutants in in vitro and in vivo assays, virulence features have been evaluated. In addition, a regulatory cross talk between adhesin determinants was found for the wild-type isolate. This particular mode of virulence regulation is missing in the mutant strain.
KW  - Escherichia coli
KW  - Genetik
Y1  - 1992
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/7157
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-71578
ER  -