TY  - JOUR
A1  - Pereira, Ana Rita
A1  - Trivanović, Drenka
A1  - Stahlhut, Philipp
A1  - Rudert, Maximilian
A1  - Groll, Jürgen
A1  - Herrmann, Marietta
T1  - Preservation of the naïve features of mesenchymal stromal cells in vitro: Comparison of cell- and bone-derived decellularized extracellular matrix
T2  - Journal of Tissue Engineering
N2  - The fate and behavior of bone marrow mesenchymal stem/stromal cells (BM-MSC) is bidirectionally influenced by their microenvironment, the stem cell niche, where a magnitude of biochemical and physical cues communicate in an extremely orchestrated way. It is known that simplified 2D in vitro systems for BM-MSC culture do not represent their naïve physiological environment. Here, we developed four different 2D cell-based decellularized matrices (dECM) and a 3D decellularized human trabecular-bone scaffold (dBone) to evaluate BM-MSC behavior. The obtained cell-derived matrices provided a reliable tool for cell shape-based analyses of typical features associated with osteogenic differentiation at high-throughput level. On the other hand, exploratory proteomics analysis identified native bone-specific proteins selectively expressed in dBone but not in dECM models. Together with its architectural complexity, the physico-chemical properties of dBone triggered the upregulation of stemness associated genes and niche-related protein expression, proving in vitro conservation of the naïve features of BM-MSC.
KW  - decellularization
KW  - bone model
KW  - stem cell niche
KW  - stemness
KW  - osteogenesis
KW  - 3D models
Y1  - 2022
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/26883
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-268835
VL  - 13
ER  -