TY  - JOUR
A1  - Dunce, James M.
A1  - Milburn, Amy E.
A1  - Gurusaran, Manickam
A1  - da Cruz, Irene
A1  - Sen, Lee T.
A1  - Benavente, Ricardo
A1  - Davies, Owen R.
T1  - Structural basis of meiotic telomere attachment to the nuclear envelope by MAJIN-TERB2-TERB1
T2  - Nature Communications
N2  - Meiotic chromosomes undergo rapid prophase movements, which are thought to facilitate the formation of inter-homologue recombination intermediates that underlie synapsis, crossing over and segregation. The meiotic telomere complex (MAJIN, TERB1, TERB2) tethers telomere ends to the nuclear envelope and transmits cytoskeletal forces via the LINC complex to drive these rapid movements. Here, we report the molecular architecture of the meiotic telomere complex through the crystal structure of MAJIN-TERB2, together with light and X-ray scattering studies of wider complexes. The MAJIN-TERB2 2:2 hetero-tetramer binds strongly to DNA and is tethered through long flexible linkers to the inner nuclear membrane and two TRF1-binding 1:1 TERB2-TERB1 complexes. Our complementary structured illumination microscopy studies and biochemical findings reveal a telomere attachment mechanism in which MAJIN-TERB2-TERB1 recruits telomere-bound TRF1, which is then displaced during pachytene, allowing MAJIN-TERB2-TERB1 to bind telomeric DNA and form a mature attachment plate.
KW  - DNA
KW  - meiosis
KW  - proteins
KW  - super-resolution microscopy
KW  - X-ray crystallography
Y1  - 2018
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/22641
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-226416
VL  - 9
ER  -