TY  - JOUR
A1  - Watermann, Christoph
A1  - Meyer, Malin Tordis
A1  - Wagner, Steffen
A1  - Wittekindt, Claus
A1  - Klussmann, Jens Peter
A1  - Erguen, Sueleyman
A1  - Baumgart-Vogt, Eveline
A1  - Karnati, Srikanth
T1  - Peroxisomes are highly abundant and heterogeneous in human parotid glands
T2  - International Journal of Molecular Sciences
N2  - The parotid gland is one of the major salivary glands producing a serous secretion, and it plays an essential role in the digestive and immune systems. Knowledge of peroxisomes in the human parotid gland is minimal; furthermore, the peroxisomal compartment and its enzyme composition in the different cell types of the human parotid gland have never been subjected to a detailed investigation. Therefore, we performed a comprehensive analysis of peroxisomes in the human parotid gland’s striated duct and acinar cells. We combined biochemical techniques with various light and electron microscopy techniques to determine the localization of parotid secretory proteins and different peroxisomal marker proteins in parotid gland tissue. Moreover, we analyzed the mRNA of numerous gene encoding proteins localized in peroxisomes using real-time quantitative PCR. The results confirm the presence of peroxisomes in all striated duct and acinar cells of the human parotid gland. Immunofluorescence analyses for various peroxisomal proteins showed a higher abundance and more intense staining in striated duct cells compared to acinar cells. Moreover, human parotid glands comprise high quantities of catalase and other antioxidative enzymes in discrete subcellular regions, suggesting their role in protection against oxidative stress. This study provides the first thorough description of parotid peroxisomes in different parotid cell types of healthy human tissue.
KW  - peroxisomes
KW  - parotid gland
KW  - human
KW  - catalase
KW  - differential expression
KW  - PSP
KW  - mRNA
KW  - immunofluorescence
Y1  - 2023
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/31107
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-311079
SN  - 1422-0067
VL  - 24
IS  - 5
ER  -