TY  - JOUR
A1  - Ammar, Mohamed Raafet
A1  - Thahouly, Tamou
A1  - Hanauer, André
A1  - Stegner, David
A1  - Nieswandt, Bernhard
A1  - Vitale, Nicolas
T1  - PLD1 participates in BDNF-induced signalling in cortical neurons
T2  - Scientific Reports
N2  - The brain-derived neurotrophic factor BDNF plays a critical role in neuronal development and the induction of L-LTP at glutamatergic synapses in several brain regions. However, the cellular and molecular mechanisms underlying these BDNF effects have not been firmly established. Using in vitro cultures of cortical neurons from knockout mice for Pld1 and Rsk2, BDNF was observed to induce a rapid RSK2-dependent activation of PLD and to stimulate BDNF ERK1/2-CREB and mTor-S6K signalling pathways, but these effects were greatly reduced in Pld1\(^{-/-}\) neurons. Furthermore, phospho-CREB did not accumulate in the nucleus, whereas overexpression of PLD1 amplified the BDNF-dependent nuclear recruitment of phospho-ERK1/2 and phospho-CREB. This BDNF retrograde signalling was prevented in cells silenced for the scaffolding protein PEA15, a protein which complexes with PLD1, ERK1/2, and RSK2 after BDNF treatment. Finally PLD1, ERK1/2, and RSK2 partially colocalized on endosomal structures, suggesting that these proteins are part of the molecular module responsible for BDNF signalling in cortical neurons.
KW  - phospholipase D
KW  - ERK map kinease
KW  - long-term potentation
KW  - brain
KW  - protein RSK2
KW  - dendritic growth
KW  - neurite outgrowth
KW  - neurotrophic factor
KW  - coffin-lowry-syndrome
KW  - phosphatidic acid
Y1  - 2015
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/13996
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-139962
VL  - 5
IS  - 14778
ER  -