TY  - JOUR
A1  - Hacker, Jörg
A1  - Knapp, S.
A1  - Goebel, W.
T1  - Spontaneous deletions and flanking regions of the chromosomal inherited hemolysin determinant of an Escherichia coli 06 strain
N2  - The hemolytic Escherichia coli strain 536 (06) propagates spontaneous hemolysin- negative mutants at relatively high rates (10-3 to 10-4 ). One type of mutant (type I) lacks both secreted (external) and periplasmic (internal) hemolysin activity (HlYex - IHlYin -) and in addition shows no mannose-resistant hemagglutination (Mrh -), whereas the other type (type II) is HlYex -IHIYin + and Mrh +. The genetic determinants for hemolysin production (hly) and for mannose-resistant hemagglutination (mrh) of this strain are located on the chromosome. Hybridization experiments with DNA probes specific for various parts of the hly determinant reveal that mutants of type I have lost the total hly determinant, whereas those of type 11 lack only part of the hlyB that is essential for transport of hemolysin across the outer membrane. Using a probe that contains the end sequence of the plasmid pHly152-encoded hly determinant (adjacent to hlyB), we determined that a related sequence flanks also the hlyB-distal end of the chromosomal hly determinant of E. coli 536. In addition several other similar or even identical sequences are found in the vicinity of the hlyC- and the hlyB-distal ends of both the chromosomal and the plasmid hly determinants.
Y1  - 1983
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/3408
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-40260
ER  -