TY  - JOUR
A1  - Altieri, Barbara
A1  - Sbiera, Silviu
A1  - Della Casa, Silvia
A1  - Weigand, Isabel
A1  - Wild, Vanessa
A1  - Steinhauer, Sonja
A1  - Fadda, Guido
A1  - Kocot, Arkadius
A1  - Bekteshi, Michaela
A1  - Mambretti, Egle M.
A1  - Rosenwald, Andreas
A1  - Pontecorvi, Alfredo
A1  - Fassnacht, Martin
A1  - Ronchi, Cristina L.
T1  - Livin/BIRC7 expression as malignancy marker in adrenocortical tumors
T2  - Oncotarget
N2  - Livin/BIRC7 is a member of the inhibitors of apoptosis proteins family, which are involved in tumor development through the inhibition of caspases. Aim was to investigate the expression of livin and other members of its pathway in adrenocortical tumors and in the adrenocortical carcinoma (ACC) cell line NCI-H295R.
The mRNA expression of livin, its isoforms α and β, XIAP, CASP3 and DIABLO was evaluated by qRT-PCR in 82 fresh-frozen adrenal tissues (34 ACC, 25 adenomas = ACA, 23 normal adrenal glands = NAG). Livin protein expression was assessed by immunohistochemistry in 270 paraffin-embedded tissues (192 ACC, 58 ACA, 20 NAG). Livin, CASP3 and cleaved caspase-3 were evaluated in NCI-H295R after induction of livin overexpression.
Relative livin mRNA expression was significantly higher in ACC than in ACA and NAG (0.060 ± 0.116 vs 0.004 ± 0.014 and 0.002 ± 0.009, respectively, p < 0.01), being consistently higher in tumors than in adjacent NAG and isoform β more expressed than α. No significant differences in CASP3, XIAP and DIABLO levels were found among these groups. In immunohistochemistry, livin was localized in both cytoplasm and nuclei. The ratio between cytoplasmic and nuclear staining was significantly higher in ACC (1.51 ± 0.66) than in ACA (0.80 ± 0.35) and NAG (0.88 ± 0.27; p < 0.0001). No significant correlations were observed between livin expression and histopathological parameters or clinical outcome. In NCI-H295R cells, the livin overexpression slightly reduced the activation of CASP3, but did not correlate with cell viability.
In conclusion, livin is specifically over-expressed in ACC, suggesting that it might be involved in adrenocortical tumorigenesis and represent a new molecular marker of malignancy.
KW  - cancer
KW  - livin
KW  - BIRC7
KW  - adrenocortical cancer
KW  - adrenal tumor
KW  - caspase-3
Y1  - 2017
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/17188
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-171887
VL  - 8
IS  - 6
ER  -