TY  - JOUR
A1  - Nanda, Indrajit
A1  - Schartl, Manfred
A1  - Feichtinger, Wolfgang
A1  - Epplen, Jörg T.
A1  - Schmid, Michael
T1  - Early stages of sex chromosome differentiation in fish as analysed by simple repetitive DNA sequences
N2  - Animal sex chromosome evolution has started on different occasions with a homologous pair of autosomes leading to morphologically differentiated gonosomes. In contrast to other vertebrate classes, among fishes cytologically dernonstrahle sex chromosomes are rare. In reptiles, certain motifs of simple tandemly repeated DNA sequences like (gata)\(_n\)/(gaca)\(_m\) are associated with the constitutive heterochromatin of sex chromosomes. In this study a panel of simple repetitive sequence probes was hybridized to restriction enzyme digested genomic DNA of poeciliid fishes. Apparent male heterogamety previously established by genetic experiments in Poecilia reticulata (guppy) was correlated with male-specific hybridization using the (GACA)\(_4\) probe. The (GATA)\(_4\) oligonucleotide identifies certain male guppies by a Y chromosomal polymorphism in the outbred population. In cantrast none of the genetically defined heterogametic situations in Xiphophorus could be verified consistently using the collection of simple repetitive sequence probes. Only individuals from particular populations produced sex-specific patterns of hybridization with (GATA)\(_4\). Additional poeciliid species (P. sphenops, P. velifera) harbour different sex-specifically organized simple repeat motifs. The observed sex-specific hybridization patterns were substantiated by banding analyses of the karyotypes and by in situ hybridization using the (GACA)\(_4\) probe.
KW  - Physiologische Chemie
Y1  - 1992
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/5366
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-61715
ER  -