TY  - JOUR
A1  - Voss, Lena J.
A1  - McAdam, Scott A. M.
A1  - Knoblauch, Michael
A1  - Rathje, Jan M.
A1  - Brodribb, Tim
A1  - Hedrich, Rainer
A1  - Roelfsema, M. Rob G.
T1  - Guard cells in fern stomata are connected by plasmodesmata, but control cytosolic Ca2+ levels autonomously
T2  - New Phytologist
N2  - Recent studies have revealed that some responses of fern stomata to environmental signals differ from those of their relatives in seed plants. However, it is unknown whether the biophysical properties of guard cells differ fundamentally between species of both clades.

Intracellular micro-electrodes and the fluorescent Ca2+ reporter FURA2 were used to study voltage-dependent cation channels and Ca2+ signals in guard cells of the ferns Polypodium vulgare and Asplenium scolopendrium.

Voltage clamp experiments with fern guard cells revealed similar properties of voltage-dependent K+ channels as found in seed plants. However, fluorescent dyes moved within the fern stomata, from one guard cell to the other, which does not occur in most seed plants. Despite the presence of plasmodesmata, which interconnect fern guard cells, Ca2+ signals could be elicited in each of the cells individually.

Based on the common properties of voltage-dependent channels in ferns and seed plants, it is likely that these key transport proteins are conserved in vascular plants. However, the symplastic connections between fern guard cells in mature stomata indicate that the biophysical mechanisms that control stomatal movements differ between ferns and seed plants.
KW  - calcium signals
KW  - ferns
KW  - guard cell
KW  - plasmodesmata
KW  - potassium channels
KW  - seed plants
KW  - stomata
Y1  - 2018
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/23324
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-233247
VL  - 219
ER  -