TY  - JOUR
A1  - Heckmann, Manfred
A1  - Pauli, Martin
T1  - Visualizing presynaptic active zones and synaptic vesicles
T2  - Frontiers in Synaptic Neuroscience
N2  - The presynaptic active zone (AZ) of chemical synapses is a highly dynamic compartment where synaptic vesicle fusion and neurotransmitter release take place. During evolution the AZ was optimized for speed, accuracy, and reliability of chemical synaptic transmission in combination with miniaturization and plasticity. Single-molecule localization microscopy (SMLM) offers nanometer spatial resolution as well as information about copy number, localization, and orientation of proteins of interest in AZs. This type of imaging allows quantifications of activity dependent AZ reorganizations, e.g., in the context of presynaptic homeostatic potentiation. In combination with high-pressure freezing and optogenetic or electrical stimulation AZs can be imaged with millisecond temporal resolution during synaptic activity. Therefore SMLM allows the determination of key parameters in the complex spatial environment of AZs, necessary for next generation simulations of chemical synapses with realistic protein arrangements.
KW  - active zone
KW  - depression
KW  - facilitation
KW  - plasticity
KW  - potentiation
KW  - synapse
Y1  - 2022
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/27468
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-274687
SN  - 1663-3563
VL  - 14
ER  -