TY  - JOUR
A1  - Schmalzl, Jonas
A1  - Plumhoff, Piet
A1  - Gilbert, Fabian
A1  - Gohlke, Frank
A1  - Konrads, Christian
A1  - Brunner, Ulrich
A1  - Jakob, Franz
A1  - Ebert, Regina
A1  - Steinert, Andre F.
T1  - Tendon-derived stem cells from the long head of the biceps tendon
T2  - Bone & Joint Research
N2  - Objectives
The long head of the biceps (LHB) is often resected in shoulder surgery and could therefore serve as a cell source for tissue engineering approaches in the shoulder. However, whether it represents a suitable cell source for regenerative approaches, both in the inflamed and non-inflamed states, remains unclear. In the present study, inflamed and native human LHBs were comparatively characterized for features of regeneration.

Methods
In total, 22 resected LHB tendons were classified into inflamed samples (n = 11) and non-inflamed samples (n = 11). Proliferation potential and specific marker gene expression of primary LHB-derived cell cultures were analyzed. Multipotentiality, including osteogenic, adipogenic, chondrogenic, and tenogenic differentiation potential of both groups were compared under respective lineage-specific culture conditions.

Results
Inflammation does not seem to affect the proliferation rate of the isolated tendon-derived stem cells (TDSCs) and the tenogenic marker gene expression. Cells from both groups showed an equivalent osteogenic, adipogenic, chondrogenic and tenogenic differentiation potential in histology and real-time polymerase chain reaction (RT-PCR) analysis.

Conclusion
These results suggest that the LHB tendon might be a suitable cell source for regenerative approaches, both in inflamed and non-inflamed states. The LHB with and without tendinitis has been characterized as a novel source of TDSCs, which might facilitate treatment of degeneration and induction of regeneration in shoulder surgery.
KW  - biceps tendon
KW  - tendon-derived stem cell
KW  - mesenchymal stem cell
KW  - tissue engineering
KW  - shoulder
Y1  - 2019
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/20037
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-200370
VL  - 8
IS  - 9
ER  -