TY  - JOUR
A1  - Niklass, Solveig
A1  - Stoyanov, Stoyan
A1  - Garz, Cornelia
A1  - Bueche, Celine Z.
A1  - Mencl, Stine
A1  - Reymann, Klaus
A1  - Heinze, Hans-Jochen
A1  - Carare, Roxana O.
A1  - Kleinschnitz, Christoph
A1  - Schreiber, Stefanie
T1  - Intravital imaging in spontaneously hypertensive stroke-prone rats-a pilot study
T2  - Experimental & Translational Stroke Medicine
N2  - Background
There is growing evidence that endothelial failure and subsequent blood brain barrier (BBB) breakdown initiate cerebral small vessel disease (CSVD) pathology. In spontaneously hypertensive stroke-prone rats (SHRSP) endothelial damage is indicated by intraluminal accumulations of erythrocytes (erythrocyte thrombi) that are not observed with current magnetic resonance imaging techniques. Two-photon microscopy (2 PM) offers the potential for real-time direct detection of the small vasculature. Thus, within this pilot study we investigated the sensitivity of 2 PM to detect erythrocyte thrombi expressing initiating CSVD phenomena in vivo.

Methods
Eight SHRSP and 13 Wistar controls were used for in vivo imaging and subsequent histology with haematoxylin-eosin (HE). For 2 PM, cerebral blood vessels were labeled by fluorescent Dextran (70 kDa) applied intraorbitally. The correlation between vascular erythrocyte thrombi observed by 2 PM and HE-staining was assessed. Artificial surgical damage and parenchymal Dextran distribution were analyzed postmortem.

Results
Dextran was distributed within the small vessel walls and co-localized with IgG.
Artificial surgical damage was comparable between SHRSP and Wistar controls and mainly affected the small vasculature. In fewer than 20% of animals there was correlation between erythrocyte thrombi as observed with 2 PM and histologically with HE.

Conclusions
Contrary to our initial expectations, there was little agreement between intravital 2 PM imaging and histology for the detection of erythrocyte thrombi. Two-photon microscopy is a valuable technique that complements but does not replace the value of conventional histology.
KW  - SHRSP
KW  - Intravital imaging
KW  - 2 PM
KW  - CSVD
Y1  - 2014
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/12135
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-121353
VL  - 6
ER  -