TY  - JOUR
A1  - Busch, Albert
A1  - Busch, Martin
A1  - Scholz, Claus-Jürgen
A1  - Kellersmann, Richard
A1  - Otto, Christoph
A1  - Chernogubova, Ekaterina
A1  - Maegdefessel, Lars
A1  - Zernecke, Alma
A1  - Lorenz, Udo
T1  - Aneurysm miRNA Signature Differs, Depending on Disease Localization and Morphology
T2  - International Journal of Molecular Science
N2  - Limited comprehension of aneurysm pathology has led to inconclusive results from clinical trials. miRNAs are key regulators of post-translational gene modification and are useful tools in elucidating key features of aneurysm pathogenesis in distinct entities of abdominal and popliteal aneurysms. Here, surgically harvested specimens from 19 abdominal aortic aneurysm (AAA) and 8 popliteal artery aneurysm (PAA) patients were analyzed for miRNA expression and histologically classified regarding extracellular matrix (ECM) remodeling and inflammation. DIANA-based computational target prediction and pathway enrichment analysis verified our results, as well as previous ones. miRNA-362, -19b-1, -194, -769, -21 and -550 were significantly down-regulated in AAA samples depending on degree of inflammation. Similar or inverse regulation was found for miR-769, 19b-1 and miR-550, -21, whereas miR-194 and -362 were unaltered in PAA. In situ hybridization verified higher expression of miR-550 and -21 in PAA compared to AAA and computational analysis for target genes and pathway enrichment affirmed signal transduction, cell-cell-interaction and cell degradation pathways, in line with previous results. Despite the vague role of miRNAs for potential diagnostic and treatment purposes, the number of candidates from tissue signature studies is increasing. Tissue morphology influences subsequent research, yet comparison of distinct entities of aneurysm disease can unravel core pathways.
KW  - AAA
KW  - miRNA expression
KW  - pathway analysis
KW  - histologic diversity
KW  - popliteal aneurysm
Y1  - 2016
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/14642
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-146422
SN  - International Journal of Molecular Science
VL  - 17
IS  - 1
ER  -