TY  - JOUR
A1  - Bury, Susanne
A1  - Soundararajan, Manonmani
A1  - Bharti, Richa
A1  - von Bünau, Rudolf
A1  - Förstner, Konrad U.
A1  - Oelschlaeger, Tobias A.
T1  - The probiotic escherichia coli strain Nissle 1917 combats lambdoid bacteriophages stx and lambda
T2  - Frontiers in Microbiology
N2  - Shiga toxin (Stx) producing E. coli (STEC) such as Enterohemorrhagic E. coli (EHEC) are the major cause of foodborne illness in humans. In vitro studies showed the probiotic Escherichia coil strain Nissle 1917 (EcN) to efficiently inhibit the production of Stx. Life threatening EHEC strains as for example the serotype 0104:H4, responsible for the great outbreak in 2011 in Germany, evolutionary developed from certain E. coll strains which got infected by stx2-encoding lambdoid phages turning the E. coil into lysogenic and subsequently Stx producing strains. Since antibiotics induce stx genes and Stx production, EHEC infected persons are not recommended to be treated with antibiotics. Therefore, EcN might be an alternative medication. However, because even commensal E. coli strains might be converted into Stx-producers after becoming host to a stx encoding prophage, we tested EcN for stx-phage genome integration. Our experiments revealed the resistance of EcN toward not only stx-phages but also against lambda-phages. This resistance was not based on the lack of or by mutated phage receptors. Rather it involved the expression of a phage repressor (pr) gene of a defective prophage in EcN which was able to partially protect E. coli K-12 strain MG1655 against stx and lambda phage infection. Furthermore, we observed EcN to inactivate phages and thereby to protect E. coli K-12 strains against infection by stx- as well as lambda-phages. Inactivation of lambda-phages was due to binding of lambda-phages to LamB of EcN whereas inactivation of stx-phages was caused by a thermostable protein of EcN. These properties together with its ability to inhibit Stx production make EcN a good candidate for the prevention of illness caused by EHEC and probably for the treatment of already infected people.
KW  - probiotic
KW  - E. coli Nissle 1917
KW  - EHEC
KW  - Shiga toxin producing E. coli
KW  - stx-phages
KW  - lambda-phages
KW  - lambdoid prophage
KW  - LamB
Y1  - 2018
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/22196
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-221960
VL  - 9
ER  -