TY  - JOUR
A1  - Kreft, Jürgen
A1  - Burger, Klaus J.
A1  - Goebel, Werner
T1  - Expression of antibiotic resistance genes from Escherichia coli in Bacillus subtilis
N2  - Bifunctional recombinant plasmids were constructed, comprised of the E. coli vectors pBR322, pBR325 and pACYC184 and different plasmids from Gram-positive bacteria, e.g. pBSU161-1 of B. subtilis and pUB110 and pC221 of S. aureus. The beta-lactamase (bla) gene and the chloramphenicol acetyltransferase (cat) gene from the E. coli plasmids were not transcribed and therefore not expressed in B. subtilis. However, tetracycline resistance from the E. coli plasmids was expressed in B. subtilis. Transcription of the tetracycline resistance gene(s) started in B. subtilis at or near the original E. coli promoter, the sequence of which is almost identical with the sequence recognized by σ<sup>55</sup> of B. subtilis RNA polymerase.
KW  - Biologie
Y1  - 1983
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/5049
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-60600
ER  -