TY  - JOUR
A1  - Scheer, Ulrich
A1  - Hansmann, Paul
A1  - Falk, Heinz
A1  - Sitte, Peter
T1  - Ultrastructural localization of DNA in two Cryptomonas species by use of a monoclonal DNA-antibody
N2  - Immunogold cytochemistry - DNA localization - Cryptomonas nucleomorph The distribution and subcellular localization of DNA in the unicellular alga Cryptomonas has been investigated electron-microscopically by indirect immunocytochemistry, using a monoclonal DNA antibody and a gold-Iabeled secondary antibody. This technique proved to be very sensitive and entirely specific. DNA could be demonstrated in four different compartments (nucleus, nucleomorph, plastid, and mitochondrion). Within the plastid, DNA is concentrated in stroma regions that are localized preferentially around the center of the organelle. The mitochondrion contains several isolated DNA-containing regions (nucleoids). Within the nucleus, most of the DNA is localized in the 'condensed' chromatin. DNA was also detectable in small areas of the nucleolus, whereas the interchromatin space of the nucleus appeared almost devoid of DNA. Within the nucleomorph, DNA is distributed inhomogeneously in the matrix. DNA could furthermore be detected in restricted areas of the 'fibrillogranular body' of the nucleomorph, resembling the situation encountered in the nucleol us. The presence of DNA and its characteristic distribution in the nucleomorph provide additional, strong evidence in favour of the interpretation of that organelle as the residual nucleus of a eukaryotic endosymbiont in Cryptomonas.
KW  - Cytologie
Y1  - 1986
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/3333
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-39746
ER  -