TY  - JOUR
A1  - Bakari-Soale, Majeed
A1  - Ikenga, Nonso Josephat
A1  - Scheibe, Marion
A1  - Butter, Falk
A1  - Jones, Nicola G.
A1  - Kramer, Susanne
A1  - Engstler, Markus
T1  - The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei
T2  - Scientific Reports
N2  - The biosynthesis of ribosomes is a complex cellular process involving ribosomal RNA, ribosomal proteins and several further trans-acting factors. DExD/H box proteins constitute the largest family of trans-acting protein factors involved in this process. Several members of this protein family have been directly implicated in ribosome biogenesis in yeast. In trypanosomes, ribosome biogenesis differs in several features from the process described in yeast. Here, we have identified the DExD/H box helicase Hel66 as being involved in ribosome biogenesis. The protein is unique to Kinetoplastida, localises to the nucleolus and its depletion via RNAi caused a severe growth defect. Loss of the protein resulted in a decrease of global translation and accumulation of rRNA processing intermediates for both the small and large ribosomal subunits. Only a few factors involved in trypanosome rRNA biogenesis have been described so far and our findings contribute to gaining a more comprehensive picture of this essential process.
KW  - infection
KW  - parasite evolution
KW  - parasite genetics
KW  - RNA
Y1  - 2021
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/26387
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-263872
VL  - 11
IS  - 1
ER  -