TY  - JOUR
A1  - Ascierto, Maria Libera
A1  - Worschech, Andrea
A1  - Yu, Zhiya
A1  - Adams, Sharon
A1  - Reinboth, Jennifer
A1  - Chen, Nanhai G
A1  - Pos, Zoltan
A1  - Roychoudhuri, Rahul
A1  - Di Pasquale, Giovanni
A1  - Bedognetti, Davide
A1  - Uccellini, Lorenzo
A1  - Rossano, Fabio
A1  - Ascierto, Paolo A
A1  - Stroncek, David F
A1  - Restifo, Nicholas P
A1  - Wang, Ena
A1  - Szalay, Aladar A
A1  - Marincola, Francesco M
T1  - Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68
T2  - BMC Cancer
N2  - Background: 

Oncolytic viral therapy represents an alternative therapeutic strategy for the treatment of cancer. We previously described GLV-1h68, a modified Vaccinia Virus with exclusive tropism for tumor cells, and we observed a cell line-specific relationship between the ability of GLV-1h68 to replicate in vitro and its ability to colonize and eliminate tumor in vivo. 

Methods: 

In the current study we surveyed the in vitro permissivity to GLV-1h68 replication of the NCI-60 panel of cell lines. Selected cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain. In order to identify correlates of permissity to viral infection, we measured transcriptional profiles of the cell lines prior infection. 

Results: 

We observed highly heterogeneous permissivity to VACV infection amongst the cell lines. The heterogeneity of permissivity was independent of tissue with the exception of B cell derivation. Cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain and a significant correlation was found suggesting a common permissive phenotype. While no clear transcriptional pattern could be identified as predictor of permissivity to infection, some associations were observed suggesting multifactorial basis permissivity to viral infection. 

Conclusions: 

Our findings have implications for the design of oncolytic therapies for cancer and offer insights into the nature of permissivity of tumor cells to viral infection.
KW  - gene-therapy
KW  - adenovirus
KW  - receptor
KW  - identification
KW  - infection
KW  - CD9
KW  - panel
Y1  - 2011
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/14150
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-141503
VL  - 11
IS  - 451
ER  -