TY  - JOUR
A1  - Hacker, Jörg
A1  - Hughes, C.
A1  - Hof, H.
A1  - Goebel, W.
T1  - Cloned hemolysin genes from Escherichia coli that cause urinary tract infection determine different levels of toxicity in mice
N2  - After intraperitoneal injection of mice with Escherichia coli strains isolated from patients with urinary tract infections, the mortality due to hemolytic (Hly+) and nonhemolytic (Hiy-) isolates was 77 and 40%, respectively. Deletion of the chromosomal hemolysin (h/y) determinant in an E. co/i 06:K15:H31 urinary tract infection strain led to a significant reduction in toxicity for mice, and its reintroduction on a recombinant plasmid partially restored the original toxicity. Although introduction of the cloned plasmid pHiy152-encoded hly determinant into the Hly- E. coli 06 mutant strain increased toxicity by only a marginal degree, transformation with the cloned chromosomal hly determinants from two E. coli strains of serotypes 018ac:K5:H- and 075:K95:H? resulted in markedly greater toxicity, even exceeding that of the original Hly+ E. coli 06 wild-type strain.
KW  - Infektionsbiologie
Y1  - 1983
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/5234
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-59330
ER  -