TY  - JOUR
A1  - Zdziarski, Jaroslaw
A1  - Brzuszkiewicz, Elzbieta
A1  - Wullt, Bjorn
A1  - Liesegang, Heiko
A1  - Biran, Dvora
A1  - Voigt, Birgit
A1  - Gronberg-Hernandez, Jenny
A1  - Ragnarsdottir, Bryndis
A1  - Hecker, Michael
A1  - Ron, Eliora Z.
A1  - Daniel, Rolf
A1  - Gottschalk, Gerhard
A1  - Hacker, Joerg
A1  - Svanborg, Catharina
A1  - Dobrindt, Ulrich
T1  - Host Imprints on Bacterial Genomes-Rapid, Divergent Evolution in Individual Patients
N2  - Bacteria lose or gain genetic material and through selection, new variants become fixed in the population. Here we provide the first, genome-wide example of a single bacterial strain’s evolution in different deliberately colonized patients and the surprising insight that hosts appear to personalize their microflora. By first obtaining the complete genome sequence of the prototype asymptomatic bacteriuria strain E. coli 83972 and then resequencing its descendants after therapeutic bladder colonization of different patients, we identified 34 mutations, which affected metabolic and virulence-related genes. Further transcriptome and proteome analysis proved that these genome changes altered bacterial gene expression resulting in unique adaptation patterns in each patient. Our results provide evidence that, in addition to stochastic events, adaptive bacterial evolution is driven by individual host environments. Ongoing loss of gene function supports the hypothesis that evolution towards commensalism rather than virulence is favored during asymptomatic bladder colonization.
KW  - Proteomanalyse
KW  - Bakterien
Y1  - 2010
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/5744
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-68594
ER  -