TY  - JOUR
A1  - Doppler, Kathrin
A1  - Appeltshauser, Luise
A1  - Krämer, Heidrun H.
A1  - King Man Ng, Judy
A1  - Meinl, Edgar
A1  - Villmann, Carmen
A1  - Brophy, Peter
A1  - Dib-Hajj, Sulayman D.
A1  - Waxman, Stephen G.
A1  - Weishaupt, Andreas
A1  - Sommer, Claudia
T1  - Contactin-1 and Neurofascin-155/-186 Are Not Targets of Auto-Antibodies in Multifocal Motor Neuropathy
T2  - PLoS One
N2  - Multifocal motor neuropathy is an immune mediated disease presenting with multifocal muscle weakness and conduction block. IgM auto-antibodies against the ganglioside GM1 are detectable in about 50% of the patients. Auto-antibodies against the paranodal proteins contactin-1 and neurofascin-155 and the nodal protein neurofascin-186 have been detected in subgroups of patients with chronic inflammatory demyelinating polyneuropathy. Recently, auto-antibodies against neurofascin-186 and gliomedin were described in more than 60% of patients with multifocal motor neuropathy. In the current study, we aimed to validate this finding, using a combination of different assays for auto-antibody detection. In addition we intended to detect further auto-antibodies against paranodal proteins, specifically contactin-1 and neurofascin-155 in multifocal motor neuropathy patients’ sera. We analyzed sera of 33 patients with well-characterized multifocal motor neuropathy for IgM or IgG anti-contactin-1, anti-neurofascin-155 or -186 antibodies using enzyme-linked immunosorbent assay, binding assays with transfected human embryonic kidney 293 cells and murine teased fibers. We did not detect any IgM or IgG auto-antibodies against contactin-1, neurofascin-155 or -186 in any of our multifocal motor neuropathy patients. We conclude that auto-antibodies against contactin-1, neurofascin-155 and -186 do not play a relevant role in the pathogenesis in this cohort with multifocal motor neuropathy.
KW  - motor proteins
KW  - enzyme-linked immunoassays
KW  - binding analysis
KW  - neuropathy
KW  - nerve fibers
KW  - cell binding assay
KW  - antibodies
KW  - enzyme assays
Y1  - 2015
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/12615
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-126156
VL  - 10
IS  - 7
ER  -