TY  - JOUR
A1  - Reimann, Hauke
A1  - Stopper, Helga
A1  - Hintzsche, Henning
T1  - Long-term fate of etoposide-induced micronuclei and micronucleated cells in Hela-H2B-GFP cells
T2  - Archives of Toxicology
N2  - Micronuclei are small nuclear cellular structures containing whole chromosomes or chromosomal fragments. While there is a lot of information available about the origin and formation of micronuclei, less is known about the fate of micronuclei and micronucleated cells. Possible fates include extrusion, degradation, reincorporation and persistence. Live cell imaging was performed to quantitatively analyse the fates of micronuclei and micronucleated cells occurring in vitro. Imaging was conducted for up to 96 h in HeLa-H2B-GFP cells treated with 0.5, 1 and 2 µg/ml etoposide. While a minority of micronuclei was reincorporated into the main nucleus during mitosis, the majority of micronuclei persisted without any alterations. Degradation and extrusion were observed rarely or never. The presence of micronuclei affected the proliferation of the daughter cells and also had an influence on cell death rates. Mitotic errors were found to be clearly increased in micronucleus-containing cells. The results show that micronuclei and micronucleated cells can, although delayed in cell cycle, sustain for multiple divisions.
KW  - micronuclei
KW  - cell fate
KW  - etoposide
KW  - live imaging
KW  - DNA damage
Y1  - 2020
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/23503
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-235039
SN  - 0340-5761
VL  - 94
ER  -