TY  - JOUR
A1  - Kobsar, Anna
A1  - Koehnlechner, Karina
A1  - Klingler, Philipp
A1  - Niklaus, Marius
A1  - Zeller-Hahn, Julia
A1  - Koessler, Angela
A1  - Weber, Katja
A1  - Boeck, Markus
A1  - Koessler, Juergen
T1  - The effect of short-term refrigeration on platelet responsiveness
T2  - Scientific Reports
N2  - Storage of platelet concentrates (PC) at cold temperature (CT) is discussed as an alternative to the current standard of storage at room temperature (RT). Recently, we could show that cold-induced attenuation of inhibitory signaling is an important mechanism promoting platelet reactivity. For developing strategies in blood banking, it is required to elucidate the time-dependent onset of facilitated platelet activation. Thus, freshly prepared platelet-rich-plasma (PRP) was stored for 1 and 2 h at CT (2–6 °C) or at RT (20–24 °C), followed by subsequent comparative analysis. Compared to RT, basal and induced vasodilator-stimulated phosphoprotein (VASP) phosphorylation levels were decreased under CT within 1 h by approximately 20%, determined by Western blot analysis and flow cytometry. Concomitantly, ADP- and collagen-induced threshold aggregation values were enhanced by up to 30–40%. Furthermore, platelet-covered areas on collagen-coated slides and aggregate formation under flow conditions were increased after storage at CT, in addition to induced activation markers. In conclusion, a time period of 1–2 h for refrigeration is sufficient to induce an attenuation of inhibitory signaling, accompanied with an enhancement of platelet responsiveness. Short-term refrigeration may be considered as a rational approach to obtain PC with higher functional reactivity for the treatment of hemorrhage.
KW  - short‑term refrigeration
KW  - platelet responsiveness
KW  - cold temperature
Y1  - 2022
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/30139
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-301390
VL  - 12
IS  - 1
ER  -