TY  - JOUR
A1  - Hahn, Lukas
A1  - Beudert, Matthias
A1  - Gutmann, Marcus
A1  - Keßler, Larissa
A1  - Stahlhut, Philipp
A1  - Fischer, Lena
A1  - Karakaya, Emine
A1  - Lorson, Thomas
A1  - Thievessen, Ingo
A1  - Detsch, Rainer
A1  - Lühmann, Tessa
A1  - Luxenhofer, Robert
T1  - From Thermogelling Hydrogels toward Functional Bioinks: Controlled Modification and Cytocompatible Crosslinking
T2  - Macromolecular Bioscience
N2  - Hydrogels are key components in bioink formulations to ensure printability and stability in biofabrication. In this study, a well-known Diels-Alder two-step post-polymerization modification approach is introduced into thermogelling diblock copolymers, comprising poly(2-methyl-2-oxazoline) and thermoresponsive poly(2-n-propyl-2-oxazine). The diblock copolymers are partially hydrolyzed and subsequently modified by acid/amine coupling with furan and maleimide moieties. While the thermogelling and shear-thinning properties allow excellent printability, trigger-less cell-friendly Diels-Alder click-chemistry yields long-term shape-fidelity. The introduced platform enables easy incorporation of cell-binding moieties (RGD-peptide) for cellular interaction. The hydrogel is functionalized with RGD-peptides using thiol-maleimide chemistry and cell proliferation as well as morphology of fibroblasts seeded on top of the hydrogels confirm the cell adhesion facilitated by the peptides. Finally, bioink formulations are tested for biocompatibility by incorporating fibroblasts homogenously inside the polymer solution pre-printing. After the printing and crosslinking process good cytocompatibility is confirmed. The established bioink system combines a two-step approach by physical precursor gelation followed by an additional chemical stabilization, offering a broad versatility for further biomechanical adaptation or bioresponsive peptide modification.
KW  - chemical crosslinking
KW  - biofabrication
KW  - bioprinting
KW  - hydrogels
Y1  - 2021
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/25754
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-257542
VL  - 21
IS  - 10
ER  -