TY  - JOUR
A1  - Bencurova, Elena
A1  - Shityakov, Sergey
A1  - Schaack, Dominik
A1  - Kaltdorf, Martin
A1  - Sarukhanyan, Edita
A1  - Hilgarth, Alexander
A1  - Rath, Christin
A1  - Montenegro, Sergio
A1  - Roth, Günter
A1  - Lopez, Daniel
A1  - Dandekar, Thomas
T1  - Nanocellulose composites as smart devices with chassis, light-directed DNA Storage, engineered electronic properties, and chip integration
T2  - Frontiers in Bioengineering and Biotechnology
N2  - The rapid development of green and sustainable materials opens up new possibilities in the field of applied research. Such materials include nanocellulose composites that can integrate many components into composites and provide a good chassis for smart devices. In our study, we evaluate four approaches for turning a nanocellulose composite into an information storage or processing device: 1) nanocellulose can be a suitable carrier material and protect information stored in DNA. 2) Nucleotide-processing enzymes (polymerase and exonuclease) can be controlled by light after fusing them with light-gating domains; nucleotide substrate specificity can be changed by mutation or pH change (read-in and read-out of the information). 3) Semiconductors and electronic capabilities can be achieved: we show that nanocellulose is rendered electronic by iodine treatment replacing silicon including microstructures. Nanocellulose semiconductor properties are measured, and the resulting potential including single-electron transistors (SET) and their properties are modeled. Electric current can also be transported by DNA through G-quadruplex DNA molecules; these as well as classical silicon semiconductors can easily be integrated into the nanocellulose composite. 4) To elaborate upon miniaturization and integration for a smart nanocellulose chip device, we demonstrate pH-sensitive dyes in nanocellulose, nanopore creation, and kinase micropatterning on bacterial membranes as well as digital PCR micro-wells. Future application potential includes nano-3D printing and fast molecular processors (e.g., SETs) integrated with DNA storage and conventional electronics. This would also lead to environment-friendly nanocellulose chips for information processing as well as smart nanocellulose composites for biomedical applications and nano-factories.
KW  - nanocellulose
KW  - DNA storage
KW  - light-gated proteins
KW  - single-electron transistors
KW  - protein chip
Y1  - 2022
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/28303
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-283033
SN  - 2296-4185
VL  - 10
ER  -