TY  - JOUR
A1  - El-Hawary, Seham S.
A1  - Sayed, Ahmed M.
A1  - Mohammed, Rabab
A1  - Hassan, Hossam M.
A1  - Rateb, Mostafa E.
A1  - Amin, Elham
A1  - Mohammed, Tarek A.
A1  - El-Mesery, Mohamed
A1  - Bin Muhsinah, Abdullatif
A1  - Alsayari, Abdulrhman
A1  - Wajant, Harald
A1  - Anany, Mohamed A.
A1  - Abdelmohsen, Usama Ramadan
T1  - Bioactive brominated oxindole alkaloids from the Red Sea sponge Callyspongia siphonella
T2  - Marine Drugs
N2  - In the present study, LC-HRESIMS-assisted dereplication along with bioactivity-guided isolation led to targeting two brominated oxindole alkaloids (compounds 1 and 2) which probably play a key role in the previously reported antibacterial, antibiofilm, and cytotoxicity of Callyspongia siphonella crude extracts. Both metabolites showed potent antibacterial activity against Gram-positive bacteria, Staphylococcus aureus (minimum inhibitory concentration (MIC) = 8 and 4 µg/mL) and Bacillus subtilis (MIC = 16 and 4 µg/mL), respectively. Furthermore, they displayed moderate biofilm inhibitory activity in Pseudomonas aeruginosa (49.32% and 41.76% inhibition, respectively), and moderate in vitro antitrypanosomal activity (13.47 and 10.27 µM, respectively). In addition, they revealed a strong cytotoxic effect toward different human cancer cell lines, supposedly through induction of necrosis. This study sheds light on the possible role of these metabolites (compounds 1 and 2) in keeping fouling organisms away from the sponge outer surface, and the possible applications of these defensive molecules in the development of new anti-infective agents.
KW  - Callyspongia siphonella
KW  - LC-HRESIMS
KW  - metabolomic profiling
KW  - oxindole alkaloids
KW  - tisindoline
KW  - antibacterial
KW  - antibiofilm
KW  - antitrypanosomal
KW  - anticancer
Y1  - 2019
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/20148
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-201485
VL  - 17
IS  - 8
ER  -