TY  - JOUR
A1  - Calderon, Dayana
A1  - Peña, Luis
A1  - Suarez, Angélica
A1  - Villamil, Carolina
A1  - Ramirez-Rojas, Adan
A1  - Anzola, Juan M.
A1  - García-Betancur, Juan C.
A1  - Cepeda, Martha L.
A1  - Uribe, Daniel
A1  - Del Portillo, Patricia
A1  - Mongui, Alvaro
T1  - Recovery and functional validation of hidden soil enzymes in metagenomic libraries
T2  - MicrobiologyOpen
N2  - The vast microbial diversity on the planet represents an invaluable source for identifying novel activities with potential industrial and therapeutic application. In this regard, metagenomics has emerged as a group of strategies that have significantly facilitated the analysis of DNA from multiple environments and has expanded the limits of known microbial diversity. However, the functional characterization of enzymes, metabolites, and products encoded by diverse microbial genomes is limited by the inefficient heterologous expression of foreign genes. We have implemented a pipeline that combines NGS and Sanger sequencing as a way to identify fosmids within metagenomic libraries. This strategy facilitated the identification of putative proteins, subcloning of targeted genes and preliminary characterization of selected proteins. Overall, the in silico approach followed by the experimental validation allowed us to efficiently recover the activity of previously hidden enzymes derived from agricultural soil samples. Therefore, the methodology workflow described herein can be applied to recover activities encoded by environmental DNA from multiple sources.
KW  - environmental microbiology
KW  - functional genomics
KW  - metagenomics
KW  - microbial genomics
Y1  - 2019
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/22201
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-222016
VL  - 8
ER  -