TY  - JOUR
A1  - Fazeli, Gholamreza
A1  - Stetter, Maurice
A1  - Lisack, Jaime N.
A1  - Wehman, Ann M.
T1  - C. elegans Blastomeres Clear the Corpse of the Second Polar Body by LC3-Associated Phagocytosis
T2  - Cell Reports
N2  - To understand how undifferentiated pluripotent cells cope with cell corpses, we examined the clearance of polar bodies born during female meiosis. We found that polar bodies lose membrane integrity and expose phosphatidylserine in Caenorhabditis elegans. Polar body signaling recruits engulfment receptors to the plasma membrane of embryonic blastomeres using the PI3K VPS-34, RAB-5 GTPase and the sorting nexin SNX-6. The second polar body is then phagocytosed using receptor-mediated engulfment pathways dependent on the Rac1 ortholog CED-10 but undergoes non-apoptotic programmed cell death independent of engulfment. RAB-7 GTPase is required for lysosome recruitment to the polar body phagosome, while LC3 lipidation is required for degradation of the corpse membrane after lysosome fusion. The polar body phagolysosome vesiculates in an mTOR- and ARL-8-dependent manner, which assists its timely degradation. Thus, we established a genetic model to study clearance by LC3-associated phagocytosis and reveal insights into the mechanisms of phagosome maturation and degradation.
KW  - cell corpse clearance
KW  - phagosome maturation
KW  - LC3-associated phagocytosis
KW  - lysosomal degradation
KW  - phagolysosome tubulation
KW  - polar body
KW  - non-canonical autophagy
KW  - non-apoptotic programmed cell death
Y1  - 2018
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/22765
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-227651
VL  - 23
ER  -