TY  - JOUR
A1  - Rubtsov, P. M.
A1  - Oganessyan, R. G.
A1  - Gorboulev, Valentin G.
A1  - Skryabin, K. G.
A1  - Bayev, A. A.
T1  - Genetic engineering of peptide hormones : II. Possible polymorphism of preprolactin in cattle. Data of molecular cloning
N2  - Primary structure is determined of an insertion of a clone isolated from the library of hypophyseal cDNA of cattle by hybridization with a probe specific for prolactin. Analysis of nucleotide sequences showed that in the process of cloning, reorganization occurred in structure of preprolactin cDNA, including an inversion of the 5'-terminal and deletion of the central section of cDNA. Nevertheless, from structure of cDNA, nucleotide sequences can be deduced of extended 5'- and 3'-terminal sections of preprolactin mRNA in cattle with lengths of 257 and 551 nucleotide residues, respectively. When these sequences are compared to those established previously, some differences were found in primary structure. The most important of them is the presence of an additional codon which codes alanine at the position (-22) of the signal peptide. It is suggested that heterogeneity of preprolactin mRNA of cattle in the section coding the signal peptide is the result of alternative splicing, as was shown for preprolactin mRNA in rats.
Y1  - 1988
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/4080
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-46975
ER  -