TY  - JOUR
A1  - Heiser, A.
A1  - Ulrichs, Karin
A1  - Müller-Ruchholtz, W.
T1  - Prophylactic trypsin inhibition during the isolation procedure guarantees reproducible, high porcine islet yields
N2  - Abstract: For isolating islets from the porcine pancreas, we established a semiautomated digestion method. Although the isolation technique was standardized and collagenase of controlled quality was used, until now the reproducibility of high islet yields was unsatisfactory. Our hypothesis was that pancreatic trypsin was responsible for this failure. The aim of this study was to investigate the effect of endogenous trypsin on islet yield. Our results demonstrate that a high trypsin level correlates with poor islet yield, whereas low trypsin activity always correlates with high islet yield. Specific inhibition of trypsin results in low trypsin activity and reproducible, high islet yields.
KW  - islets of Langerhans
KW  - xenogenic transplantation
KW  - swine
KW  - enzyme activation
KW  - enzyme inhibitors
Y1  - 1994
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/4229
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-45531
ER  -