TY  - JOUR
A1  - Rethwilm, Axel
A1  - Erlwein, Otto
A1  - Baunach, Gerald
A1  - Mauerer, Bernd
A1  - ter Meulen, Volker
T1  - The transcriptional transactivator of human foamy virus maps to the bel 1 genomic region
N2  - The human foamy virus (HFV) genome possesses three open reading frames (bel I, 2, and 3) located between env and the 3' long terminal repeat. By analogy to other human retroviruses this region was selected as the most Iikely candidate to encode the viral transactivator. ResuIts presented here confirmed this and showed further that a deletion introduced only into the bell open reading frame of a plasmid derived from an infectious molecular clone of HFV abolished transactivation. In contrast, deletions in bel 2 and bel 3 had only minor effects on the ability to transactivate. The role of the bel I genomic region as a transactivator was further investigated by eukaryotic expression of a genome fragment of HFV spanning the bel I open reading frame. A construct expressing bell under control of a heterologous promoter was found to transactivate the HFV long terminal repeat in a dose-dependent fashion. Furthermore, it is shown that the U3 region of the HFV long terminal repeat is sufficient to respond to the HFV transactivator.
KW  - Virologie
Y1  - 1991
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/4512
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-47342
ER  -