TY  - JOUR
A1  - Ute, Hentschel
A1  - Reisberg, Eva E.
A1  - Hildebrandt, Ulrich
A1  - Riederer, Markus
T1  - Distinct Phyllosphere Bacterial Communities on Arabidopsis Wax Mutant Leaves
T2  - PLoS ONE
N2  - The phyllosphere of plants is inhabited by diverse microorganisms, however, the factors shaping their community composition are not fully elucidated. The plant cuticle represents the initial contact surface between microorganisms and the plant. We thus aimed to investigate whether mutations in the cuticular wax biosynthesis would affect the diversity of the phyllosphere microbiota. A set of four Arabidopsis thaliana eceriferum mutants (cer1, cer6, cer9, cer16) and their respective wild type (Landsberg erecta) were subjected to an outdoor growth period and analysed towards this purpose. The chemical distinctness of the mutant wax phenotypes was confirmed by gas chromatographic measurements. Next generation amplicon pyrosequencing of the bacterial communities showed distinct community patterns. This observation was supported by denaturing gradient gel electrophoresis experiments. Microbial community analyses revealed bacterial phylotypes that were ubiquitously present on all plant lines (termed “core” community) while others were positively or negatively affected by the wax mutant phenotype (termed “plant line-specific“ community). We conclude from this study that plant cuticular wax composition can affect the community composition of phyllosphere bacteria.
KW  - arabidopsis thaliana
KW  - bacteria
KW  - community structure
KW  - denaturing gradient gel electrophoresis
KW  - fatty acids
KW  - leaves
KW  - plant communities
KW  - waxes
Y1  - 2013
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/9669
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-96699
ER  -