TY  - JOUR
A1  - Calebiro, Davide
A1  - Maiellaro, Isabella
T1  - cAMP signaling microdomains and their observation by optical methods
T2  - Frontiers in Cellular Neuroscience
N2  - The second messenger cyclic AMP (cAMP) is a major intracellular mediator of many hormones and neurotransmitters and regulates a myriad of cell functions, including synaptic plasticity in neurons. Whereas cAMP can freely diffuse in the cytosol, a growing body of evidence suggests the formation of cAMP gradients and microdomains near the sites of cAMP production, where cAMP signals remain apparently confined. The mechanisms responsible for the formation of such microdomains are subject of intensive investigation. The development of optical methods based on fluorescence resonance energy transfer (FRET), which allow a direct observation of cAMP signaling with high temporal and spatial resolution, is playing a fundamental role in elucidating the nature of such microdomains. Here, we will review the optical methods used for monitoring cAMP and protein kinase A (PKA) signaling in living cells, providing some examples of their application in neurons, and will discuss the major hypotheses on the formation of cAMP/PKA microdomains.
KW  - G protein-coupled receptor
KW  - cyclic AMP
KW  - signaling microdomain
KW  - fluorescence resonance energy transfer
KW  - neurons
Y1  - 2014
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/11825
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-118252
SN  - 1662-5102
VL  - 8
ER  -