TY  - JOUR
A1  - Temme, Sebastian
A1  - Friebe, Daniela
A1  - Schmidt, Timo
A1  - Poschmann, Gereon
A1  - Hesse, Julia
A1  - Steckel, Bodo
A1  - Stühler, Kai
A1  - Kunz, Meik
A1  - Dandekar, Thomas
A1  - Ding, Zhaoping
A1  - Akhyari, Payam
A1  - Lichtenberg, Artur
A1  - Schrader, Jürgen
T1  - Genetic profiling and surface proteome analysis of human atrial stromal cells and rat ventricular epicardium-derived cells reveals novel insights into their cardiogenic potential
T2  - Stem Cell Research
N2  - Epicardium-derived cells (EPDC) and atrial stromal cells (ASC) display cardio-regenerative potential, but the molecular details are still unexplored. Signals which induce activation, migration and differentiation of these cells are largely unknown. Here we have isolated rat ventricular EPDC and rat/human ASC and performed genetic and proteomic profiling. EPDC and ASC expressed epicardial/mesenchymal markers (WT-1, Tbx18, CD73,CD90, CD44, CD105), cardiac markers (Gata4, Tbx5, troponin T) and also contained phosphocreatine. We used cell surface biotinylation to isolate plasma membrane proteins of rEPDC and hASC, Nano-liquid chromatography with subsequent mass spectrometry and bioinformatics analysis identified 396 rat and 239 human plasma membrane proteins with 149 overlapping proteins. Functional GO-term analysis revealed several significantly enriched categories related to extracellular matrix (ECM), cell migration/differentiation, immunology or angiogenesis. We identified receptors for ephrin and growth factors (IGF, PDGF, EGF, anthrax toxin) known to be involved in cardiac repair and regeneration. Functional category enrichment identified clusters around integrins, PI3K/Akt-signaling and various cardiomyopathies. Our study indicates that EPDC and ASC have a similar molecular phenotype related to cardiac healing/regeneration. The cell surface proteome repository will help to further unravel the molecular details of their cardio-regenerative potential and their role in cardiac diseases.
KW  - Biology
KW  - Epicardium-derived cells
KW  - Human atrial stromal cells
KW  - Cell surface proteomics
Y1  - 2017
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/17271
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-172716
VL  - 25
ER  -