TY  - JOUR
A1  - Bachmann, Julia
A1  - Ehlert, Elias
A1  - Becker, Matthias
A1  - Otto, Christoph
A1  - Radeloff, Katrin
A1  - Blunk, Torsten
A1  - Bauer-Kreisel, Petra
T1  - Ischemia-like stress conditions stimulate trophic activities of adipose-derived stromal/stem cells
T2  - Cells
N2  - Adipose-derived stromal/stem cells (ASCs) have been shown to exert regenerative functions, which are mainly attributed to the secretion of trophic factors. Upon transplantation, ASCs are facing an ischemic environment characterized by oxygen and nutrient deprivation. However, current knowledge on the secretion capacity of ASCs under such conditions is limited. Thus, the present study focused on the secretory function of ASCs under glucose and oxygen deprivation as major components of ischemia. After exposure to glucose/oxygen deprivation, ASCs maintained distinct viability, but the metabolic activity was greatly reduced by glucose limitation. ASCs were able to secrete a broad panel of factors under glucose/oxygen deprivation as revealed by a cytokine antibody array. Quantification of selected factors by ELISA demonstrated that glucose deprivation in combination with hypoxia led to markedly higher secretion levels of the angiogenic and anti-apoptotic factors IL-6, VEGF, and stanniocalcin-1 as compared to the hypoxic condition alone. A conditioned medium of glucose/oxygen-deprived ASCs promoted the viability and tube formation of endothelial cells, and the proliferation and migration of fibroblasts. These findings indicate that ASCs are stimulated by ischemia-like stress conditions to secrete trophic factors and would be able to exert their beneficial function in an ischemic environment.
KW  - adipose-derived stromal/stem cells (ASCs)
KW  - regenerative medicine
KW  - secretion
KW  - trophic factors
KW  - ischemia
KW  - glucose starvation
KW  - hypoxia
Y1  - 2020
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/21123
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-211233
SN  - 2073-4409
VL  - 9
IS  - 9
ER  -