TY  - JOUR
A1  - Geisinger, Adriana
A1  - Rodríguez-Casuriaga, Rosana
A1  - Benavente, Ricardo
T1  - Transcriptomics of Meiosis in the Male Mouse
T2  - Frontiers in Cell and Developmental Biology
N2  - Molecular studies of meiosis in mammals have been long relegated due to some intrinsic obstacles, namely the impossibility to reproduce the process in vitro, and the difficulty to obtain highly pure isolated cells of the different meiotic stages. In the recent years, some technical advances, from the improvement of flow cytometry sorting protocols to single-cell RNAseq, are enabling to profile the transcriptome and its fluctuations along the meiotic process. In this mini-review we will outline the diverse methodological approaches that have been employed, and some of the main findings that have started to arise from these studies. As for practical reasons most studies have been carried out in males, and mostly using mouse as a model, our focus will be on murine male meiosis, although also including specific comments about humans. Particularly, we will center on the controversy about gene expression during early meiotic prophase; the widespread existing gap between transcription and translation in meiotic cells; the expression patterns and potential roles of meiotic long non-coding RNAs; and the visualization of meiotic sex chromosome inactivation from the RNAseq perspective.
KW  - meiosis
KW  - transcriptomics
KW  - RNAseq
KW  - meiotic prophase
KW  - spermatogenesis
KW  - lncRNAs
KW  - MSCI
KW  - spermatogenic cell sorting
Y1  - 2021
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/23103
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-231032
SN  - 2296-634X
VL  - 9
ER  -