TY  - JOUR
A1  - Franke, Maximilian
A1  - Bieber, Michael
A1  - Stoll, Guido
A1  - Schuhmann, Michael Klaus
T1  - Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence
T2  - Methods and Protocols
N2  - The clinical and preclinical research of ischemic strokes (IS) is becoming increasingly comprehensive, especially with the emerging evidence of complex thrombotic and inflammatory interactions. Within these, the blood brain barrier (BBB) plays an important role in regulating the cellular interactions at the vascular interface and is therefore the object of many IS-related questions. Consequently, valid, economic and responsible methods to define BBB integrity are necessary. Therefore, we compared the three ex-vivo setups albumin Western blot (WB), IgG WB and albumin intensity measurement (AIM) with regard to validity as well as temporal and economic efficacy. While the informative value of the three methods correlated significantly, the efficacy of the IgG WB dominated.
KW  - IgG
KW  - albumin
KW  - immunohistochemistry
KW  - Western blot
KW  - stroke
KW  - tMCAO
KW  - blood brain barrier
KW  - neuroinflammation
Y1  - 2021
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/23421
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-234214
SN  - 2409-9279
VL  - 4
IS  - 1
ER  -