TY  - JOUR
A1  - Zhou, Yang
A1  - Ding, Meiqi
A1  - Duan, Xiaodong
A1  - Konrad, Kai R.
A1  - Nagel, Georg
A1  - Gao, Shiqiang
T1  - Extending the Anion Channelrhodopsin-Based Toolbox for Plant Optogenetics
T2  - Membranes
N2  - Optogenetics was developed in the field of neuroscience and is most commonly using light-sensitive rhodopsins to control the neural activities. Lately, we have expanded this technique into plant science by co-expression of a chloroplast-targeted β-carotene dioxygenase and an improved anion channelrhodopsin GtACR1 from the green alga Guillardia theta. The growth of Nicotiana tabacum pollen tube can then be manipulated by localized green light illumination. To extend the application of analogous optogenetic tools in the pollen tube system, we engineered another two ACRs, GtACR2, and ZipACR, which have different action spectra, light sensitivity and kinetic features, and characterized them in Xenopus laevis oocytes, Nicotiana benthamiana leaves and N. tabacum pollen tubes. We found that the similar molecular engineering method used to improve GtACR1 also enhanced GtACR2 and ZipACR performance in Xenopus laevis oocytes. The ZipACR1 performed in N. benthamiana mesophyll cells and N. tabacum pollen tubes with faster kinetics and reduced light sensitivity, allowing for optogenetic control of anion fluxes with better temporal resolution. The reduced light sensitivity would potentially facilitate future application in plants, grown under low ambient white light, combined with an optogenetic manipulation triggered by stronger green light.
KW  - optogenetics
KW  - rhodopsin
KW  - light-sensitive anion channel
KW  - surface potential recording
KW  - pollen tube
Y1  - 2021
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/23661
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-236617
SN  - 2077-0375
VL  - 11
IS  - 4
ER  -