TY  - JOUR
A1  - Hauptstein, Julia
A1  - Forster, Leonard
A1  - Nadernezhad, Ali
A1  - Horder, Hannes
A1  - Stahlhut, Philipp
A1  - Groll, Jürgen
A1  - Blunk, Torsten
A1  - Teßmar, Jörg
T1  - Bioink Platform Utilizing Dual-Stage Crosslinking of Hyaluronic Acid Tailored for Chondrogenic Differentiation of Mesenchymal Stromal Cells
T2  - Macromolecular Bioscience
N2  - 3D bioprinting often involves application of highly concentrated polymeric bioinks to enable fabrication of stable cell-hydrogel constructs, although poor cell survival, compromised stem cell differentiation, and an inhomogeneous distribution of newly produced extracellular matrix (ECM) are frequently observed. Therefore, this study presents a bioink platform using a new versatile dual-stage crosslinking approach based on thiolated hyaluronic acid (HA-SH), which not only provides stand-alone 3D printability but also facilitates effective chondrogenic differentiation of mesenchymal stromal cells. A range of HA-SH with different molecular weights is synthesized and crosslinked with acrylated (PEG-diacryl) and allylated (PEG-diallyl) polyethylene glycol in a two-step reaction scheme. The initial Michael addition is used to achieve ink printability, followed by UV-mediated thiol–ene reaction to stabilize the printed bioink for long-term cell culture. Bioinks with high molecular weight HA-SH (>200 kDa) require comparably low polymer content to facilitate bioprinting. This leads to superior quality of cartilaginous constructs which possess a coherent ECM and a strongly increased stiffness of long-term cultured constructs. The dual-stage system may serve as an example to design platforms using two independent crosslinking reactions at one functional group, which allows adjusting printability as well as material and biological properties of bioinks.
KW  - hyaluronic acid
KW  - biofabrication
KW  - chondrogenic differentiation
KW  - dual-stage crosslinking
KW  - extracellular matrix
Y1  - 2022
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/25755
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-257556
VL  - 22
IS  - 2
ER  -