TY  - JOUR
A1  - Correia Santos, Sara
A1  - Bischler, Thorsten
A1  - Westermann, Alexander J.
A1  - Vogel, Jörg
T1  - MAPS integrates regulation of actin-targeting effector SteC into the virulence control network of Salmonella small RNA PinT
T2  - Cell Reports
N2  - A full understanding of the contribution of small RNAs (sRNAs) to bacterial virulence demands knowledge of their target suites under infection-relevant conditions. Here, we take an integrative approach to capturing targets of the Hfq-associated sRNA PinT, a known post-transcriptional timer of the two major virulence programs of Salmonella enterica. Using MS2 affinity purification and RNA sequencing (MAPS), we identify PinT ligands in bacteria under in vitro conditions mimicking specific stages of the infection cycle and in bacteria growing inside macrophages. This reveals PinT-mediated translational inhibition of the secreted effector kinase SteC, which had gone unnoticed in previous target searches. Using genetic, biochemical, and microscopic assays, we provide evidence for PinT-mediated repression of steC mRNA, eventually delaying actin rearrangements in infected host cells. Our findings support the role of PinT as a central post-transcriptional regulator in Salmonella virulence and illustrate the need for complementary methods to reveal the full target suites of sRNAs.
KW  - gene expression
KW  - nondocing RNA
KW  - chaperone HFQ
KW  - soluble-RNA
KW  - SEQ
KW  - interactome
KW  - repression
KW  - secretion
KW  - infection
KW  - biology
Y1  - 2021
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/25913
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-259134
VL  - 34
IS  - 5
ER  -