TY  - JOUR
A1  - Vitale, Maria Rosaria
A1  - Zöller, Johanna Eva Maria
A1  - Jansch, Charline
A1  - Janz, Anna
A1  - Edenhofer, Frank
A1  - Klopocki, Eva
A1  - van den Hove, Daniel
A1  - Vanmierlo, Tim
A1  - Rivero, Olga
A1  - Kasri, Nael Nadif
A1  - Ziegler, Georg Christoph
A1  - Lesch, Klaus-Peter
T1  - Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9
T2  - Stem Cell Research
N2  - Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout (CDH13\(^{+/-}\)) and a CDH13 null mutant (CDH13\(^{-/-}\)) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal female karyotype.
KW  - CRISPR-Cas Systems
KW  - cadherins
KW  - female
KW  - heterozygote
KW  - humans
KW  - Induced Pluripotent Stem Cells
KW  - middle aged
KW  - neurodevelopmental disorders / genetics
Y1  - 2021
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/26033
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-260331
VL  - 51
ER  -