TY  - CHAP
A1  - Liaqat, Anam
A1  - Sednev, Maksim V.
A1  - Höbartner, Claudia
T1  - In Vitro Selection of Deoxyribozymes for the Detection of RNA Modifications
T2  - Ribosome Biogenesis: Methods and Protocols
N2  - Deoxyribozymes are artificially evolved DNA molecules with catalytic abilities. RNA-cleaving deoxyribozymes have been recognized as an efficient tool for detection of modifications in target RNAs and provide an alternative to traditional and modern methods for detection of ribose or nucleobase methylation. However, there are only few examples of DNA enzymes that specifically reveal the presence of a certain type of modification, including N6-methyladenosine, and the knowledge about how DNA enzymes recognize modified RNAs is still extremely limited. Therefore, DNA enzymes cannot be easily engineered for the analysis of desired RNA modifications, but are instead identified by in vitro selection from random DNA libraries using synthetic modified RNA substrates. This protocol describes a general in vitro selection stagtegy to evolve new RNA-cleaving DNA enzymes that can efficiently differentiate modified RNA substrates from their unmodified counterpart.
KW  - RNA
KW  - deoxyribozymes
KW  - modified RNA nucleotides
KW  - catalytic DNA
KW  - epitranscriptomics
KW  - in vitro selection
KW  - RNA cleavage
Y1  - 2022
UR  - https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/docId/27920
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-279208
SN  - 978-1-0716-2501-9
PB  - Humana Press
ER  -